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KMID : 0894520100140010001
Development & Reproduction
2010 Volume.14 No. 1 p.1 ~ p.5
Effect of 11-ketotestosterone (11-KT) on Gonadal Sex Reversal and Spermatogenesis of Honeycomb Grouper Epinephelus merra
Lee Chi-Hoon

Hur Sang-Woo
Song Young-Bo
Takano Kazunori
Takemura Akihiro
Lee Young-Don
Abstract
We investigated the androgenic effects of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis in honeycomb grouper Epinephelus merra by method of gonadal biopsy. 11-KT was injected intramuscularly at a concentration of 1 and 10 §¶/g body weight. The proportion of cross sectional area of the gonad occupied by each germ cell type was measured and compared pre- and post-injection group. During the sex change phase, the distribution ratio of oocytes was decreased in all fish of 11-KT treatment group while the distribution ratio of spermatocytes was increased than pre-injection group. In male phase, all fish of 11-KT treatment group shown the increased distribution ratio of spermatocytes, but the distribution ratio of spermatozoa was decreased than pre-injection group. The present results suggest that 11-KT can stimulate degeneration of oocytes, proliferation of spermatocytes and spermiation in honeycomb grouper.
KEYWORD
11-KT, Honeycomb grouper, Epinephelus merra, Sex change, Gonadal biopsy
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